PROTOCOLS

STIPENDS

VIDEOS

LABORATORY PROTOCOLS

• common stock solutions and buffers

• guide to gel loading buffer & tracking dyes used in agarose gel electrophoresis

• ficoll vs. glycerol or sucrose

• commonly used tracking dyes

• relative dye mobility chart

• growth area & volume guide to tissue culture flasks, plates, and dishes

• IUPAC nucleotide symbols - what the other letters besides A, T, G, and C mean

• metric prefixes - "a picture is worth 1.66 zeptomoles of words"

• bacterial transformation - make your own ultra-competent bacteria, and heat shock for only 30 seconds for the highest transformation efficiency!

• inoculating bacteria - glass beads and bamboo skewers are your friends.

• sterilizing media in the microwave - forget the low-tech, time-consuming autoclave!

• plasmid isolation - a modified protocol for the Qiagen Mini-prep for large-scale plasmid isolation. Faster, much easier, and cheaper than the Maxi-prep!