Wendy Chao Logo

WENDY CHAO'S LABORATORY PROTOCOLS

TISSUE CULTURE AREA & VOLUME GUIDE - useful information for cell culture junkies

 
TYPE
AREA (cm2)
APPROX. CELL YIELD
WORKING VOLUME (mL)
PLATES
96 well
0.32
3.2 x 104
.100 - .200
(per well)
48 well
0.95
8.0 x 104
.190 - .285
24 well
1.9
1.9 x 105
.380 - .570
12 well
3.8
3.8 x 105
.760 - 1.14
6 well
9.5
9.5 x 105
1.9 - 2.9
DISHES
3.5 cm
8
8.0 x 105
1.6 - 2.4
6 cm
21
2.1 x 106
4.2 - 6.3
10 cm
55
5.5 x 106
11 - 16.5
FLASKS
T-25
25
2.5 x 106
5 - 7.5
T-75
75
7.5 x 106
15 - 22.5
T-175
175
1.75 x 107
35 - 52.5
adapted from Corning's Cell Culture Selection Guide
 
millions and millions
back to protocols
  wendy chao's home page

 
web www.wendychao.com