WENDY CHAO'S LABORATORY PROTOCOLS
THE WENDY-PREP - a large-scale modification of the Qiagen Spin Miniprep
The first Qiagen plasmid prep I ever performed was the Maxi Prep, and it sucked. When I got really good plasmid yield from a Qiagen mini-prep spin column, I thought, "why not just do several minipreps instead of a maxi-prep?" The miniprep spin columns take just a fraction of the time it takes to perform a standard Maxi-prep. Furthermore, the cost is about the same (or less): each Maxi-prep costs between $16-$18 (and takes HOURS), while each mini-prep costs just over $1 and only takes about 30 minutes to extract high-quality plasmid DNA.
Using the "Wendy-prep" I've isolated over 500 ug of DNA with just 8 miniprep columns and 50 mls of bacteria. I've also found that you can save a day or so of bacterial growth tme by just inoculating a 50-ml culture directly from a single colony, rather than from a "starter culture."
Large-scale plasmid isolation using the Qiagen Qiaprep Spin Miniprep Kit
Total cost: $15. Total prep time: 30 minutes.
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