WENDY CHAO'S LABORATORY PROTOCOLS
There are two ways to do most things: my way, and the wrong way.
RECIPES
GUIDES
guide
to gel loading buffer & tracking dyes used
in agarose gel electrophoresis
growth
area & volume guide to tissue culture flasks, plates, and dishes
IUPAC
nucleotide symbols - what the other letters
besides A, T, G, and C mean
metric
prefixes - "a picture is worth 1.66 zeptomoles of words"
useful
numbers & formulas - molecular weights, conversions, and all that
fun stuff
TECHNIQUES
bacterial
transformation - make your own ultra-competent bacteria, and heat
shock for only 30 seconds for the highest transformation efficiency!
inoculating
bacteria - glass beads and bamboo skewers are your friends.
sterilizing
media in the microwave - forget the low-tech, time-consuming autoclave!
plasmid
isolation - a modified protocol for the Qiagen Mini-prep for large-scale
plasmid isolation. Faster, much easier, and cheaper than the Maxi-prep!